Characterization of pathogenic roles of two Erysipelothrix rhusiopathiae surface proteins

Weifeng Zhu; Chengzhi Cai; Jingjing Huang; Liang Liu; Zhongmin Xu; Xiaomei Sun; Meilin Jin

doi:10.1016/j.micpath.2017.11.057

Characterization of pathogenic roles of two Erysipelothrix rhusiopathiae surface proteins

Microb Pathog. 2018 Jan:114:166-168. doi: 10.1016/j.micpath.2017.11.057. Epub 2017 Nov 28.

Authors

Weifeng Zhu¹, Chengzhi Cai¹, Jingjing Huang¹, Liang Liu¹, Zhongmin Xu¹, Xiaomei Sun², Meilin Jin³

Affiliations

¹ Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
² Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
³ Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. Electronic address: jml8328@126.com.

PMID: 29196173
DOI: 10.1016/j.micpath.2017.11.057

Abstract

Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. E. rhusiopathiae HP0728 and HP1472 have been reported to be down regulated in low-virulence or avirulent strains, but their pathogenic roles are not known. In this study, it was found that E. rhusiopathiae HP0728 and HP1472 were displayed on the surface of E. rhusiopathiae. Moreover, recombinant HP1472 could adhere to pig vascular endothelial cells. Recombinant HP0728 could bind host plasminogen but could not bind fibronectin. In conclusion, our work suggested that HP0728 and HP1472 are virulence factors of E. rhusiopathiae.

Keywords: Adhesin; Endothelial cell; Fibronectin; Plasminogen.

MeSH terms

Animals
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Erysipelothrix / genetics
Erysipelothrix / metabolism*
Erysipelothrix Infections / enzymology
Erysipelothrix Infections / microbiology*
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Plasminogen / metabolism
Swine
Swine Diseases / enzymology
Swine Diseases / microbiology*
Virulence
Virulence Factors / genetics
Virulence Factors / metabolism

Substances

Bacterial Proteins
Membrane Proteins
Virulence Factors
Plasminogen