Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation

Philippe E Ramos; Luís Abrunhosa; Ana Pinheiro; Miguel A Cerqueira; Carla Motta; Isabel Castanheira; Maria V Chandra-Hioe; Jayashree Arcot; José A Teixeira; António A Vicente

doi:10.1016/j.foodres.2016.10.036

Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation

Food Res Int. 2016 Dec:90:25-32. doi: 10.1016/j.foodres.2016.10.036. Epub 2016 Oct 29.

Affiliations

¹ CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: phramos@ceb.uminho.pt.
² CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
³ CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Apartado 12, 2781-901 Oeiras, Portugal.
⁴ International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
⁵ Department of Food Safety and Nutrition, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
⁶ Food Science and Technology, School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.

PMID: 29195878
DOI: 10.1016/j.foodres.2016.10.036

Abstract

This study focused on the use of a new system, an alginate|Ɛ-poly-l-lysine|alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26μg·L^-1 of folate, during 10h, and was encapsulated in the APACM. APACM proved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6logCFU·mL^-1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2g·L^-1 of lactic acid was achieved through encapsulation of L. plantarum, after 20h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively.

Keywords: 10-Formyltetrahydrofolate (PubChem CID: 168809); 5-Methyltetrahydrofolate; 5-Methyltetrahydrofolate (PubChem CID: 444412); Carbodiimide hydrochloride (PubChem CID: 2723939); Chitosan (PubChem CID: 71853); Folic acid; Folic acid (PubChem CID: 6037); Immobilization; Isothiocynate (PubChem CID: 16741); Mucoadhesive; Poly-l-sysine (PubChem CID: 53628747); Probiotic; Rhodamine B (PubChem CID: 6694); Sodium alginate (PubChem CID: 5,102,882); Tween 80 (PubChem CID: 5281955).