Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei

Wei Wang; Yumeng Chen; Dong-Zhi Wei

doi:10.1016/j.dib.2017.11.018

Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei

Data Brief. 2017 Nov 7:16:109-113. doi: 10.1016/j.dib.2017.11.018. eCollection 2018 Feb.

Authors

Wei Wang¹, Yumeng Chen¹, Dong-Zhi Wei¹

Affiliation

¹ State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, China.

Abstract

This data article contains data related to the research article entitled "Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei" (Wang et al., 2017) [1]. Four kinds of copper responsive promoters were designed. Quantitative PCR (qPCR) was performed to determine relative mRNA levels of red fluorescent protein gene (rfp) extracted from cells grown under different concentrations of CuSO₄. Three deletion vectors were constructed by using a copper-mediated self-excision cassette instead of a xylose-mediated self-excision cassette (Zhang et al., 2016) [2] to knock out xyn1, one of the two major specific endo-β-1,4-xylanases (Rauscher et al., 2006) [3], xyr1, the key transcriptional activator of cellulolytic and xylanolytic genes (Lichius et al., 2015) [4], and ace3, a factor essential for cellulase production (Häkkinen et al., 2014) [5]. This data article reports the primers, vector construction, and qPCR assay.

Keywords: Copper responsive promoter; Filamentous fungus; Gene expression; Plasmid construction; Quantitative real-time PCR; Trichoderma reesei.