Open-source do-it-yourself multi-color fluorescence smartphone microscopy

Yulung Sung; Fernando Campa; Wei-Chuan Shih

doi:10.1364/BOE.8.005075

Open-source do-it-yourself multi-color fluorescence smartphone microscopy

Biomed Opt Express. 2017 Oct 19;8(11):5075-5086. doi: 10.1364/BOE.8.005075. eCollection 2017 Nov 1.

Authors

Yulung Sung¹, Fernando Campa¹, Wei-Chuan Shih^{1

2

3

4}

Affiliations

¹ Department of Electrical & Computer Engineering, University of Houston, 4800 Calhoun Rd, Houston, TX 77204, USA.
² Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA.
³ Program of Materials Science & Engineering, University of Houston, Houston, TX 77204, USA.
⁴ Department of Chemistry, University of Houston, Houston, TX 77204, USA.

Abstract

Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of-care diagnostics. Current fluorescence smartphone microscope setups require precise illumination and imaging alignment which altogether limit its broad adoption. We report a multi-color fluorescence smartphone microscope with a single contact lens-like add-on lens and slide-launched total-internal-reflection guided illumination for three common tasks in investigative fluorescence microscopy: autofluorescence, fluorescent stains, and immunofluorescence. The open-source, simple and cost-effective design has the potential for do-it-yourself fluorescence smartphone microscopy.

Keywords: (100.1455) Blind deconvolution; (110.0180) Microscopy; (120.3620) Lens system design; (180.2520) Fluorescence microscopy; (260.6970) Total internal reflection.

Associated data

figshare/10.6084/m9.figshare.5313643