Identification of Ceratitis capitata (Diptera: Tephritidae) Sperm From Females in Traps: The Importance of the Ventral Receptacle

Abstract

The monitoring of a pest population often relies on the identification of individuals from traps. For area-wide programs utilizing the sterile insect technique, the further identification of the mated status of females found in traps is of utmost importance. For the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), this is usually verified through the assessment of sperm in the spermathecae. However, this can be misleading for species where there are other sperm storage organs such as the ventral receptacle. Here, we studied the relative importance of sperm storage in the ventral receptacle compared to the spermathecae for females from 5 to 18 d of age. Furthermore, we studied how sperm can be identified in the ventral receptacle or spermathecae after females were recovered from traps. We found no effect of female age on likelihood of sperm storage. Sperm could be identified in both sperm storage organs at 7 or 14 d after females had been placed in traps. We found that the ventral receptacle is a more reliable indicator of female mated status. Thus, we propose that if no sperm are found in the spermathecae, program managers should revise the ventral receptacle before assuming that females are not mated. This test may also be relevant to other pest tephritids that store even more sperm in the ventral receptacle than C. capitata.

Keywords: Anastrepha; Diptera; Mediterranean fruit fly; Tephritidae; spermathecae.