Isolation and Purification of Condensed Tannin from the Leaves and Branches of Prunus cerasifera and Its Structure and Bioactivities

Appl Biochem Biotechnol. 2018 Jun;185(2):464-475. doi: 10.1007/s12010-017-2635-9. Epub 2017 Nov 27.

Abstract

Prunus cerasifera has a rich resource and a weak utilization rate and its biological functions have been investigated. We found that the contents of total phenol (TP) in leaves and branches of Prunus cerasifera were 117.8 ± 8.8 and 100.04 ± 0.9 mg/g, respectively; the contents of soluble condensed tannin (SCT) were 73.95 ± 0.9 and 78.65 ± 4.1 mg/g, respectively; the structure of SCT containing afzelechin/epiafzelechin, catechin/epicatechin, and atechin/epicatechin as the main units and the SCT from leaves and branches exhibited better anti-tyrosinase and antioxidant activities. This study could clarify Prunus cerasifera condensed tannin resource availability and lay a theoretical foundation for its development as a natural antioxidant and tyrosinase inhibitor.

Keywords: Bioactivities; Enzyme kinetics; Tyrosinase.

MeSH terms

  • Antioxidants* / chemistry
  • Antioxidants* / isolation & purification
  • Enzyme Inhibitors* / chemistry
  • Enzyme Inhibitors* / isolation & purification
  • Monophenol Monooxygenase / antagonists & inhibitors*
  • Plant Leaves / chemistry*
  • Proanthocyanidins* / chemistry
  • Proanthocyanidins* / isolation & purification
  • Prunus domestica / chemistry*

Substances

  • Antioxidants
  • Enzyme Inhibitors
  • Proanthocyanidins
  • Monophenol Monooxygenase