Melatonin-Aluminum Oxide-Polymethylsiloxane Complex on Apoptosis of Liver Cells in a Model of Obesity and Type 2 Diabetes Mellitus

S V Michurina; I Yu Ischenko; S A Arkhipov; V V Klimontov; M A Cherepanova; M A Korolev; L N Rachkovskaya; E L Zav'yalov; V I Konenkov

doi:10.1007/s10517-017-3949-x

Melatonin-Aluminum Oxide-Polymethylsiloxane Complex on Apoptosis of Liver Cells in a Model of Obesity and Type 2 Diabetes Mellitus

Bull Exp Biol Med. 2017 Dec;164(2):165-169. doi: 10.1007/s10517-017-3949-x. Epub 2017 Nov 27.

Authors

S V Michurina¹, I Yu Ischenko², S A Arkhipov², V V Klimontov², M A Cherepanova², M A Korolev², L N Rachkovskaya², E L Zav'yalov³, V I Konenkov²

Affiliations

¹ Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia. s.michurina@ngs.ru.
² Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
³ Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.

PMID: 29181668
DOI: 10.1007/s10517-017-3949-x

Abstract

We studied the effects of a melatonin-aluminum oxide-polymethylsiloxane complex (complex M) on the expression of apoptosis regulators Bcl-2 and Bad in the liver of homozygous db/db BKS.Cg-Dock7^m+/+Lepr^db/J mice with obesity and type 2 diabetes. Complex M or placebo was administered daily through the gastric tube during weeks 8-16 of life. In mice with type 2 diabetes mellitus receiving placebo, enhanced immunohistochemical reactions for proapoptotic Bad protein and weak response for anti-apoptotic Bcl-2 protein were observed. Administration of complex M shifted the ratio of apoptosis regulators: the area of Bcl-2 expression significantly increased and against the background of reduced Bad expression area. These findings attest to antiapoptotic effect of complex M in the liver on the model of type 2 diabetes mellitus.

Keywords: apoptosis; diabetes mellitus type 2; liver; melatonin.

MeSH terms

Aluminum Oxide / chemistry
Animals
Apoptosis / drug effects
Blood Glucose / metabolism
Diabetes Mellitus, Type 2 / drug therapy*
Diabetes Mellitus, Type 2 / genetics
Diabetes Mellitus, Type 2 / metabolism
Diabetes Mellitus, Type 2 / pathology
Disease Models, Animal
Female
Gene Expression Regulation
Hepatocytes / drug effects*
Hepatocytes / metabolism
Hepatocytes / pathology
Homozygote
Liver / drug effects*
Liver / metabolism
Liver / pathology
Melatonin / chemistry
Melatonin / pharmacology*
Mice
Mice, Transgenic
Obesity / drug therapy*
Obesity / genetics
Obesity / metabolism
Obesity / pathology
Protective Agents / chemistry
Protective Agents / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / agonists
Proto-Oncogene Proteins c-bcl-2 / genetics
Proto-Oncogene Proteins c-bcl-2 / metabolism
Silicones / chemistry
bcl-Associated Death Protein / antagonists & inhibitors
bcl-Associated Death Protein / genetics
bcl-Associated Death Protein / metabolism

Substances

Bad protein, mouse
Blood Glucose
Protective Agents
Proto-Oncogene Proteins c-bcl-2
Silicones
bcl-Associated Death Protein
polymethylsiloxane
Bcl2 protein, mouse
Melatonin
Aluminum Oxide