Combination of MALDI-TOF MS and PBP2' latex agglutination assay for rapid MRSA detection

Marianna Ábrók; Andrea Lázár; Mária Szécsényi; Judit Deák; Edit Urbán

doi:10.1016/j.mimet.2017.11.021

Combination of MALDI-TOF MS and PBP2' latex agglutination assay for rapid MRSA detection

J Microbiol Methods. 2018 Jan:144:122-124. doi: 10.1016/j.mimet.2017.11.021. Epub 2017 Nov 24.

Authors

Marianna Ábrók¹, Andrea Lázár², Mária Szécsényi², Judit Deák², Edit Urbán²

Affiliations

¹ Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary. Electronic address: abrok.marianna@med.u-szeged.hu.
² Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.

PMID: 29180272
DOI: 10.1016/j.mimet.2017.11.021

Abstract

Sample preparation was optimized for MALDI-TOF MS directly from the selective enrichment broth to detect Staphylococcus aureus. A combination of MALDI-TOF MS and the PBP2' latex agglutination assay was applied for MRSA screening and evaluated on 255 clinical samples. MRSA colonisation can be reported already 18-24h after sample collection.

Keywords: Latex agglutination test; Methicillin-resistant Staphylococcus aureus; Penicillin-binding protein 2′; Salt-containing trypticase soy broth; Sample preparation; Screening.

MeSH terms

Humans
Latex Fixation Tests / methods
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Methicillin-Resistant Staphylococcus aureus / pathogenicity
Penicillin-Binding Proteins
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Staphylococcal Infections / diagnosis
Staphylococcus aureus / isolation & purification
Time Factors

Substances

Penicillin-Binding Proteins