Costunolide protects lipopolysaccharide/d-galactosamine-induced acute liver injury in mice by inhibiting NF-κB signaling pathway

J Surg Res. 2017 Dec:220:40-45. doi: 10.1016/j.jss.2017.06.083. Epub 2017 Jul 25.

Abstract

Background: Costunolide, a well-known sesquiterpene lactone, has been reported to have anti-inflammatory and anti-oxidative effects.

Methods: In this study, we aim to investigate the protective effects and mechanism of costunolide on lipopolysaccharide/d-galactosamine (LPS/D-Gal)-induced acute liver injury. Acute liver injury animal model was induced by intraperitoneal injection with D-Gal and LPS. Costunolide (10, 20, and 30 mg/kg) was injected intraperitoneally 1 h before or after LPS/D-Gal treatment.

Results: The results showed that costunolide significantly attenuated liver pathologic changes, as well as alanine aminotransferase and aspartate aminotransferase levels in serum. Meanwhile, costunolide inhibited the expressions of interleukin (IL-1β) and tumor necrosis factor (TNF-α) in liver tissues in a dose-dependent manner. Furthermore, costunolide dose dependently inhibited LPS/D-Gal-induced NF-κB activation.

Conclusions: In conclusion, this study suggested that costunolide could attenuate LPS/D-Gal-induced liver injury and might be a potential therapeutic reagent for liver injury.

Keywords: Acute liver injury; Costunolide; Lipopolysaccharide; NF-κB; d-galactosamine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Chemical and Drug Induced Liver Injury / blood
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Disease Models, Animal
  • Galactosamine / toxicity
  • Injections, Intraperitoneal
  • Interleukin-1beta / metabolism
  • Lipopolysaccharides / toxicity
  • Liver / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • NF-kappa B / metabolism*
  • Protective Agents / pharmacology*
  • Protective Agents / therapeutic use
  • Sesquiterpenes / pharmacology*
  • Sesquiterpenes / therapeutic use
  • Signal Transduction / drug effects*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • IL1B protein, mouse
  • Interleukin-1beta
  • Lipopolysaccharides
  • NF-kappa B
  • Protective Agents
  • Sesquiterpenes
  • Tumor Necrosis Factor-alpha
  • costunolide
  • Galactosamine
  • Aspartate Aminotransferases
  • Alanine Transaminase