Primary Colorectal Cells Culture as a Translational Research Model

Sheah Lin Lee; Nina Claire Dempsey-Hibbert; Dale Vimalachandran; Terence David Wardle; Paul A Sutton; John H H Williams

doi:10.1007/978-1-4939-7477-1_8

Primary Colorectal Cells Culture as a Translational Research Model

Methods Mol Biol. 2018:1709:97-105. doi: 10.1007/978-1-4939-7477-1_8.

Authors

Sheah Lin Lee^{1

2}, Nina Claire Dempsey-Hibbert^{1

3}, Dale Vimalachandran⁴, Terence David Wardle⁴, Paul A Sutton⁴, John H H Williams⁵

Affiliations

¹ Chester Centre for Stress Research, Institute of Medicine, University of Chester, Bache Hall, Chester, CH2 1BR, UK.
² University Hospital Southampton, Tremona Road, Southampton, SO16 6YD, UK.
³ Centre for Biomedicine Research, Manchester Metropolitan University, Chester Street, Manchester, M1 5GD, UK.
⁴ Countess of Chester Hospital, Liverpool Rd, Chester, CH2 1UL, UK.
⁵ Chester Centre for Stress Research, Institute of Medicine, University of Chester, Bache Hall, Chester, CH2 1BR, UK. John.williams@chester.ac.uk.

PMID: 29177654
DOI: 10.1007/978-1-4939-7477-1_8

Abstract

Preclinical studies are an essential stage for any pharmacological agent hoping to make its way into clinical trials. An ideal preclinical model that can accurately predict clinical response does not exist and the best that the scientific community have at the moment is to select the most relevant study model pertaining to the disease of interest from those available, which includes: cell lines, animal models, and even in-silico methodology. Currently, there is a huge gap between preclinical and clinical trial results, indicating that there is much room for improvement in developing a better model to bridge the translational gap.

Keywords: Carcinoma; Cells; Colorectal; Hsp90 inhibitors; Human; Primary; Research model; Study; Translational.

MeSH terms

Animals
Colorectal Neoplasms*
Disease Models, Animal
Humans
Primary Cell Culture / methods*
Translational Research, Biomedical / methods*