Chromogenic detection procedure for the multidrug-resistant, neonatal sepsis-associated clone Staphylococcus capitis NRCS-A

Marine Butin; Yann Dumont; Jean-Philippe Rasigade; Patricia Martins Simoes; Louise Hoden; Jean-Charles Picaud; Frédéric Laurent

doi:10.1016/j.diagmicrobio.2017.10.012

Chromogenic detection procedure for the multidrug-resistant, neonatal sepsis-associated clone Staphylococcus capitis NRCS-A

Diagn Microbiol Infect Dis. 2018 Feb;90(2):81-82. doi: 10.1016/j.diagmicrobio.2017.10.012. Epub 2017 Oct 23.

Authors

Marine Butin¹, Yann Dumont², Jean-Philippe Rasigade³, Patricia Martins Simoes³, Louise Hoden², Jean-Charles Picaud⁴, Frédéric Laurent⁵

Affiliations

¹ International Center of Research in Infectiology, INSERM U1111, CNRS UMR5308, Ecole Normale Supérieure, University of Lyon, Lyon, France; Neonatal Intensive Care Unit, Eastern Hospital Group, Hospices Civils de Lyon, Lyon, France; Institute of Infectious Agents, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France. Electronic address: marine.butin@chu-lyon.fr.
² Institute of Infectious Agents, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France.
³ International Center of Research in Infectiology, INSERM U1111, CNRS UMR5308, Ecole Normale Supérieure, University of Lyon, Lyon, France; Institute of Infectious Agents, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France.
⁴ Neonatal Intensive Care Unit, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France.
⁵ International Center of Research in Infectiology, INSERM U1111, CNRS UMR5308, Ecole Normale Supérieure, University of Lyon, Lyon, France; Institute of Infectious Agents, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France; Department of Microbiology-Mycology, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, University of Lyon, Lyon, France.

PMID: 29157953
DOI: 10.1016/j.diagmicrobio.2017.10.012

Abstract

The multiresistant Staphylococcus capitis clone NRCS-A is a major pathogen in neonates worldwide. We show that NRCS-A grows as mauve colonies with a cream-color halo after a 5-day incubation on MRSA Brilliance 2 agar (Oxoid®). This innovative protocol will ease the screening of clinical and environmental niches of this clone.

Keywords: Chromogenic medium; MRSA; Neonatal ICUs; Sepsis; Staphylococcus capitis.

MeSH terms

Bacteriological Techniques / methods*
Chromogenic Compounds / analysis*
Chromogenic Compounds / metabolism
Feces / microbiology
Female
Humans
Infant, Newborn
Intensive Care, Neonatal
Neonatal Sepsis / diagnosis
Neonatal Sepsis / microbiology*
Pregnancy
Skin / microbiology
Staphylococcal Infections / diagnosis
Staphylococcal Infections / microbiology*
Staphylococcus capitis / isolation & purification*
Staphylococcus capitis / metabolism
Vagina / microbiology

Substances

Chromogenic Compounds