A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways

Lihua Hou; Meng Meng; Yuanyuan Chen; Chunling Wang

doi:10.18632/oncotarget.21252

A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways

Oncotarget. 2017 Sep 23;8(49):86604-86614. doi: 10.18632/oncotarget.21252. eCollection 2017 Oct 17.

Authors

Lihua Hou¹, Meng Meng¹, Yuanyuan Chen¹, Chunling Wang¹

Affiliation

¹ Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.

Abstract

Here, the immunomodulatory effects of water-soluble polysaccharide from Grifola frondosa on RAW264.7 macrophages and its molecular mechanisms were investigated. G. frondosa polysaccharide could obviously enhance immunostimulatory activity such as the release of nitric oxide and cytokine production. Western blotting results showed that G. frondosa polysaccharide elevated the TLR4, which might act as an upstream regulator of MyD88 induced G. frondosa polysaccharide. MyD88 promoted IKKβ in endochylema and translocate NF-κB p65 subunit into the nucleus which increased the NO production and cytokine/chemokines level. The results suggested that G. frondosa polysaccharide activated macrophages through TLR4-MyD88-IKKβ-NF-κBp65 signaling pathways.

Keywords: NF-κB; RAW264.7 macrophages; TLR4; immunomodulatory; water-soluble polysaccharide.