A novel protein tyrosine phosphatase like phytase from Lactobacillus fermentum NKN51: Cloning, characterization and application in mineral release for food technology applications

Rekha Sharma; Piyush Kumar; Vandana Kaushal; Rahul Das; Naveen Kumar Navani

doi:10.1016/j.biortech.2017.10.106

A novel protein tyrosine phosphatase like phytase from Lactobacillus fermentum NKN51: Cloning, characterization and application in mineral release for food technology applications

Bioresour Technol. 2018 Feb:249:1000-1008. doi: 10.1016/j.biortech.2017.10.106. Epub 2017 Nov 4.

Authors

Rekha Sharma¹, Piyush Kumar¹, Vandana Kaushal¹, Rahul Das², Naveen Kumar Navani³

Affiliations

¹ Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
² Department of Biological Sciences, Indian Institute of Science Education and Research, Kolkata 741246, India.
³ Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India. Electronic address: navnifbs@iitr.ac.in.

PMID: 29145111
DOI: 10.1016/j.biortech.2017.10.106

Abstract

A novel protein tyrosine phosphatase like phytase (PTPLP), designated as PhyLf from probiotic bacterium Lactobacillus fermentum NKN51 was identified, cloned, expressed and characterized. The recombinant PhyLf showed specific activity of 174.5 U/mg. PhyLf exhibited strict specificity towards phytate and optimum temperature at 60 °C, pH 5.0 and ionic strength of 100 mM. K_m and K_cat of PhyLf for phytate were 0.773 mM and 84.31 s^-1, respectively. PhyLf exhibited high resistance against oxidative inactivation. PhyLf shares no homology, sans the active site with reported PTLPs, warranting classification as a new subclass. Dephytinization of durum wheat and finger millet under in vitro gastrointestinal conditions using PhyLf enhanced the bioaccessibility of mineral ions. Probiotic origin, phytate specificity, resistance to oxidative environment and gastric milieu coupled with ability to release micronutrients are unique properties of PhyLf which present a strong case for its use in ameliorating nutritional value of cereals and animal feed.

Keywords: Bioaccessibility; Dephytinization; Gastrointestinal conditions; Lactobacillus fermentum NKN51; Protein tyrosine phosphatase like phytase.

MeSH terms

6-Phytase*
Animal Feed
Animals
Food Technology*
Hydrogen-Ion Concentration
Limosilactobacillus fermentum*
Minerals
Phytic Acid
Protein Tyrosine Phosphatases

Substances

Minerals
Phytic Acid
6-Phytase
Protein Tyrosine Phosphatases