Objective: To study the difference expression and diagnostic value of ribosomal protein L5 (RPL5) in papillary thyroid carcinoma (PTC) of children and adults. Methods: Realtime-PCR was performed to detect the expression of RPL5 in 22 PTC tissues and 13 pericarcinous tissues. Receiver operating characteristic (ROC) curve and Youden's index were used to evaluate the diagnostic value of RPL5 in PTC of children and adults. Results: The expression of RPL5 in PTC tissues was higher than in pericarcinous tissues. The area under curve (AUC) was 0.820 (P=0.001), and Youden's index was 0.568. The expression of RPL5 in PTC of adults was higher than children (P<0.05). The AUC and Youden's index were respectively 0.721 (P=0.069) and 0.414 in children, whereas being respectively 0.896 (P=0.0005) and 0.709 in adults. RPL5 in diagnosis of PTC of adults was better than CK19, Galectin-3 and TPO, which are commonly used for the pathologic diagnosis of PTC. Conclusion: The expression of RPL5 in PTC is higher than pericarcinous tissues, and its expression in PTC of adults is higher than children. Furthermore, PTC is a potential indicator for diagnosis of PTC.
目的： 研究核糖体蛋白L5(ribosomal protein L5，RPL5)在儿童和成人甲状腺乳头状癌中的差异表达及其诊断价值。 方法： 采用实时荧光定量PCR方法检测甲状腺乳头状癌(papillary thyroid carcinoma, PTC)癌组织和癌旁正常组织中RPL5表达情况，包括癌组织样本22例和癌旁组织样本13例(儿童甲状腺乳头状癌样本10例，癌旁组织3例；成人甲状腺乳头状癌标本12例，癌旁组织10例)。同时采用受试者工作特征曲线(receiver operating characteristic curve，ROC)中两个重要指标：曲线下面积(area under the curve，AUC)和Youden指数(Youden′s index)对RPL5在甲状腺乳头状癌中的诊断价值进行评估。 结果： 与癌旁正常组织相比，PTC肿瘤组织中RPL5表达显著增高(P＝0.001)，同时成人PTC肿瘤组织中RPL5表达显著高于儿童PTC(P＝0.044)。RPL5诊断成人和儿童PTC的AUC＝0.820(P＝0.001)，Youden指数＝0.568；儿童PTC对应的AUC＝0.721(P＝0.069)，Youden指数＝0.414，而在成人分别为AUC＝0.896(P＝0.000 5)，Youden指数＝0.709)，明显高于儿童PTC。进一步分析发现，RPL5在成人PTC的AUC(0.896，Youden指数0.709)要好于目前临床常用指标CK19(AUC＝ 0.857，Yoden指数0.774)、Galectin－3(AUC 0.868，Youden指数0.775)和甲状腺过氧化物酶(AUC 0.830，Youden指数0.698)。 结论： 本研究阐明RPL5在PTC肿瘤中表达显著高于癌旁组织，并明确RPL5对PTC具诊断价值。此外，本研究还提示RPL5作为分子标志物在成人PTC中的诊断价值明显高于儿童PTC，有助于提高PTC临床诊断。.
Keywords: Carcinoma, papillary; Diagnostic value; Receiver operating characteristic curve; Ribosomal proteins; Thyroid neoplasms.