Effect of water activity and temperature on the growth of Eurotium species isolated from animal feeds

Abstract

Background: Xerophilic fungi represent a serious problem due to their ability to grow at low water activities causing the spoiling of low and intermediate moisture foods, stored goods and animal feeds, with the consequent economic losses.

Aims: The combined effect of water activity and temperature of four Eurotium species isolated from animal feeds was investigated.

Methods: Eurotium amstelodami, Eurotium chevalieri, Eurotium repens and Eurotium rubrum were grown at 5, 15, 25, 37 and 45°C on malt extract agar adjusted with glycerol in the range 0.710-0.993 of water activities.

Results: The cardinal model proposed by Rosso and Robinson (2001) was applied to fit growth data, with the variable water activity at fixed temperatures, obtaining three cardinal water activities (a_wmin, a_wmax, a_wopt) and the specific growth rate at the optimum a_w (μ_opt). A probabilistic model was also applied to define the interface between growth and no-growth. The cardinal model provided an adequate estimation of the optimal a_w to grow and the maximum growth rate. The probabilistic model showed a good performance to fit growth/no-growth cases in the predicted range.

Conclusions: The results presented here could be applied to predict Eurotium species growth in animal feeds.

Keywords: Actividad del agua; Animal feeds; Crecimiento; Eurotium; Growth; Piensos para animales; Temperatura; Temperature; Water activity.