Electro-acoustic sensor for the real-time identification of the bacteriophages

О I Guliy; B D Zaitsev; I A Borodina; А М Shikhabudinov; S А Staroverov; L A Dykman; A S Fomin

doi:10.1016/j.talanta.2017.10.020

Electro-acoustic sensor for the real-time identification of the bacteriophages

Talanta. 2018 Feb 1:178:743-750. doi: 10.1016/j.talanta.2017.10.020. Epub 2017 Oct 12.

Authors

О I Guliy¹, B D Zaitsev², I A Borodina², А М Shikhabudinov², S А Staroverov³, L A Dykman⁴, A S Fomin⁴

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia; Saratov State Vavilov Agrarian University, Saratov 410012, Russia; Scientific Research Veterinary Institute, Russian Academy of Sciences, Saratov 410028, Russia. Electronic address: guliy_olga@mail.ru.
² Kotel'nikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov Branch, Saratov 410019, Russia.
³ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia; Saratov State Vavilov Agrarian University, Saratov 410012, Russia; Scientific Research Veterinary Institute, Russian Academy of Sciences, Saratov 410028, Russia.
⁴ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia; Scientific Research Veterinary Institute, Russian Academy of Sciences, Saratov 410028, Russia.

PMID: 29136890
DOI: 10.1016/j.talanta.2017.10.020

Abstract

The electro-acoustical sensor for the real-time identification of the bacteriophage FAl-SR65 is described. The polyclonal antibodies specific to the bacteriophage FAl-SR65 were for the first time obtained, and the possibility of their application to the bacteriophage detection by using the electro-acoustical method of analysis was also studied for the first time. The electro-acoustical sensor was based on the lateral electric field excited piezoelectric resonator. The frequency dependences of the real and imaginary parts of the electrical impedance of such a resonator, loaded with the virus-antibody suspension, significantly differed from those measured for the same resonator with the same virus suspension but without antibodies (control). The limit for the reliable bacteriophage detection during their interaction with the antibodies (sensitivity level) was determined to be 10⁶ phages ml^-1. The obtained results demonstrate the possibility to record the bacteriophage-antibody interactions and can be used as the basis for the development of the biological sensor for the quantitative virus detection.

Keywords: Antibodies; Detection of bacteriophages; Electro-acoustical analysis.

MeSH terms

Acoustics*
Bacteriophages / isolation & purification*
Electrochemistry / instrumentation*
Time Factors