Practical Advice on the One-Step Growth Curve

Andrew M Kropinski

doi:10.1007/978-1-4939-7343-9_3

Practical Advice on the One-Step Growth Curve

Methods Mol Biol. 2018:1681:41-47. doi: 10.1007/978-1-4939-7343-9_3.

Author

Andrew M Kropinski^{1

2

3}

Affiliations

¹ Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1. akropins@uoguelph.ca.
² Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1. akropins@uoguelph.ca.
³ Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1. akropins@uoguelph.ca.

PMID: 29134585
DOI: 10.1007/978-1-4939-7343-9_3

Abstract

The one-step growth experiment is fundamental to the description of a new bacteriophage. The following protocol is optimized for those working with rapidly growing bacterial cultures.

Keywords: Adsorption; Burst size; Latent period; One-step growth.

MeSH terms

Bacteria / virology
Bacteriophages / growth & development*
Microbiological Techniques / methods*
Statistics as Topic