Imaging cellular trafficking processes in real time using lysosome targeted up-conversion nanoparticles

Sumit Kumar Pramanik; Sreejesh Sreedharan; Harwinder Singh; Nicola H Green; Carl Smythe; Jim A Thomas; Amitava Das

doi:10.1039/c7cc08185e

Imaging cellular trafficking processes in real time using lysosome targeted up-conversion nanoparticles

Chem Commun (Camb). 2017 Nov 23;53(94):12672-12675. doi: 10.1039/c7cc08185e.

Authors

Sumit Kumar Pramanik¹, Sreejesh Sreedharan, Harwinder Singh, Nicola H Green, Carl Smythe, Jim A Thomas, Amitava Das

Affiliation

¹ CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India. sumitpramanik@csmcri.res.in a.das@csmcri.res.in.

PMID: 29131207
DOI: 10.1039/c7cc08185e

Abstract

β-NaYF₄:Yb,Gd up-conversion nanoparticles, UCNPs, surface functionalized with suitable targetting peptides function as nontoxic lysosome-specific imaging probes.

MeSH terms

Animals
Cell Survival / drug effects
Cell Tracking*
Fluorescent Dyes / chemistry
Fluorescent Dyes / pharmacology
Fluorides / chemistry
Fluorides / pharmacology
Gadolinium / chemistry
Gadolinium / pharmacology
Lysosomes / metabolism*
Mice
Nanoparticles / chemistry*
Optical Imaging / methods*
Particle Size
RAW 264.7 Cells
Surface Properties
Time Factors
Ytterbium / chemistry
Ytterbium / pharmacology
Yttrium / chemistry
Yttrium / pharmacology

Substances

Fluorescent Dyes
sodium yttriumtetrafluoride
Yttrium
Gadolinium
Ytterbium
Fluorides