Photo-triggered recognition between host and guest compounds in a giant vesicle encapsulating photo-pierceable vesicles

Chem Phys Lipids. 2018 Jan:210:70-75. doi: 10.1016/j.chemphyslip.2017.11.008. Epub 2017 Nov 9.

Abstract

Here, we used centrifugal precipitation to construct a giant vesicle (GV) encapsulating smaller giant vesicles (GV-in-GV) which comprises a photo-resistant outer GV and a photo-pierceable inner GV; the outer GV contained a fluorescent probe (SYBR Green I) in its inner water pool, and the inner GV contained double-stranded DNA (dsDNA) in its inner water pool. The phospholipid membrane of the inner GV was made photo-pierceable by inclusion of ca. 15mol% of a caged phospholipid in its membrane. Immediately after exposure of the GV-in-GVs to UV irradiation, strong fluorescence was detected in the inner water pool of the outer GV, indicating that dsDNA had been released from the inner GV and had complexed with the fluorescent probe. These dynamics can be recognized as a macroscopic representation of the molecular level function of a caged compound.

Keywords: Caged compound; Fluorescence probe; Giant vesicle; Photo-pierceable GV; Photo-triggered recognition; SYBR Green I; Vesosome; dsDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • Lipid Bilayers / chemistry*
  • Molecular Structure
  • Particle Size
  • Phospholipids / chemistry*
  • Photochemical Processes
  • Surface Properties
  • Water / chemistry*

Substances

  • Fluorescent Dyes
  • Lipid Bilayers
  • Phospholipids
  • Water
  • DNA