Flow Cytometric Evaluation of Primary Immunodeficiencies

Andreas Boldt; Michael Bitar; Ulrich Sack

doi:10.1016/j.cll.2017.07.013

Flow Cytometric Evaluation of Primary Immunodeficiencies

Clin Lab Med. 2017 Dec;37(4):895-913. doi: 10.1016/j.cll.2017.07.013.

Authors

Andreas Boldt¹, Michael Bitar², Ulrich Sack²

Affiliations

¹ Medical Faculty, Department of Diagnostics, Institute of Clinical Immunology, University of Leipzig, Johannisallee 30, Leipzig D-04103, Germany. Electronic address: Andreas.boldt@medizin.uni-leipzig.de.
² Medical Faculty, Department of Diagnostics, Institute of Clinical Immunology, University of Leipzig, Johannisallee 30, Leipzig D-04103, Germany.

PMID: 29128075
DOI: 10.1016/j.cll.2017.07.013

Abstract

Primary immunodeficiency diseases are genetic disorders that mostly cause susceptibility to infections and are sometimes associated with autoimmune and malignant diseases. For early detection and management of these diseases, flow cytometric procedures allow an encompassing assessment of cellular phenotypes and cellular functions. State-of-the art cytometry is based today on 8- to 10-color staining and includes an assessment of lineage maturation and functional markers.

Keywords: B-cell defect; Flow cytometry; Immunodeficiency; Immunophenotyping; T-cell defect.

Publication types

Review

MeSH terms

Flow Cytometry*
Humans
Immunologic Deficiency Syndromes / diagnosis*
Immunophenotyping