Hidden Secrets of Sigma54 Promoters Revealed

Nan Hao; Keith E Shearwin

doi:10.1016/j.tibs.2017.10.005

Hidden Secrets of Sigma54 Promoters Revealed

Trends Biochem Sci. 2017 Dec;42(12):931-932. doi: 10.1016/j.tibs.2017.10.005. Epub 2017 Nov 7.

Authors

Nan Hao¹, Keith E Shearwin²

Affiliations

¹ Department of Molecular and Cellular Biology, School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
² Department of Molecular and Cellular Biology, School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia. Electronic address: keith.shearwin@adelaide.edu.au.

PMID: 29126750
DOI: 10.1016/j.tibs.2017.10.005

Abstract

Bacterial sigma54 (σ⁵⁴) promoters are the DNA-binding motif for σ⁵⁴-containing RNA polymerase (RNAP) holoenzymes. A recent study using a combination of synthetic oligonucleotide library screening, biochemical characterization, and bioinformatics has uncovered a new and unexpected role for σ⁵⁴ promoters, encoding a form of bacterial 'insulator sequence' to dampen unwanted translation.

Keywords: flow-seq; oligonucleotide library screen; σ(54) promoter.

Publication types

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MeSH terms

Bacterial Proteins / genetics
DNA-Binding Proteins
DNA-Directed RNA Polymerases / genetics
Promoter Regions, Genetic
RNA Polymerase Sigma 54*
Sigma Factor / genetics*

Substances

Bacterial Proteins
DNA-Binding Proteins
Sigma Factor
DNA-Directed RNA Polymerases
RNA Polymerase Sigma 54