Targeted secondary metabolic and physico-chemical traits analysis to assess genetic variability within a germplasm collection of "long storage" tomatoes

Food Chem. 2018 Apr 1:244:275-283. doi: 10.1016/j.foodchem.2017.10.043. Epub 2017 Oct 10.

Abstract

"Long storage" tomato is a crop traditionally cultivated in the Mediterranean area under no water supply, that recently has attracted the interest of breeders for its high tolerance to drought and as potential genetic source in breeding programs for water stress resistance. A collection of 28 genotypes of "long storage" tomato (Solanum lycopersicum L.) was studied for carotenoid and polyphenol profile and content, vitamin C, and other physico-chemical traits of fruits. Tomato carotenoids and polyphenols were identified and quantified using high-performance liquid chromatography coupled with diode array detection and electrospray-mass spectrometry (HPLC/DAD/ESI-MS); nineteen different phenolic compounds and six different carotenoids, for a total of 25 markers, have been detected, quantified and used to discriminate among the different landraces to find out which could be the best candidate for a medium-to-large scale cultivation. Different statistical approaches (ANOVA, Principal Components Analysis, Cluster Analysis) have been used for data analysis.

Keywords: Carotenoids; Chlorogenic acid (PubChem CID: 1794427); Drought resistance; Lycopene (PubChem CID: 446925); Naringenin (PubChem CID: 932); Neochlorogenic acid (PubChem CID: 5280633); Polyphenols; Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805); Solanum lycopersicum L.; Targeted metabolic analysis; trans-Lutein (PubChem CID: 5281243).; β-Carotene (PubMed CID: 5280489); “Long storage” genotypes.

MeSH terms

  • Ascorbic Acid / analysis
  • Carotenoids / analysis
  • Chemical Phenomena*
  • Food Storage*
  • Fruit / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Genetic Variation*
  • Polyphenols / analysis
  • Solanum lycopersicum / chemistry*
  • Solanum lycopersicum / genetics
  • Solanum lycopersicum / metabolism*

Substances

  • Polyphenols
  • Carotenoids
  • Ascorbic Acid