Determination of the Bacteriophage Host Range: Culture-Based Approach

Andrey V Letarov; Eugene E Kulikov

doi:10.1007/978-1-4939-7395-8_7

Determination of the Bacteriophage Host Range: Culture-Based Approach

Methods Mol Biol. 2018:1693:75-84. doi: 10.1007/978-1-4939-7395-8_7.

Authors

Andrey V Letarov^{1

2

3}, Eugene E Kulikov^{4

5}

Affiliations

¹ Winogradsky Institute of Microbiology RC Biotechnology RAS, Leninski pr. 33, Build. 2, Moscow, 119071, Russia. letarov@gmail.com.
² Lomonosov Moscow State University, Leninskie Gory 1, Moscow, 119991, Russia. letarov@gmail.com.
³ Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141700, Russia. letarov@gmail.com.
⁴ Winogradsky Institute of Microbiology RC Biotechnology RAS, Leninski pr. 33, Build. 2, Moscow, 119071, Russia.
⁵ Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141700, Russia.

PMID: 29119433
DOI: 10.1007/978-1-4939-7395-8_7

Abstract

The bacteriophage host range is one of the most practically important characteristics of each bacterial virus. Here the classical plate-culture-based approach for bacteriophage host range determination is described. The important considerations related to interpretation of the data and limitations of the methods are discussed.

Keywords: Efficiency of plating; Host range; Infectivity; Resistance; Titer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / virology*
Bacteriophages / physiology*
Host Specificity*
Host-Pathogen Interactions