Can 9q34.2 rs633862 polymorphism predict survival in epithelial ovarian cancer?

Rong Jiang; Yuan Xu; Pan Wang; Xi Cheng; Tingyan Shi; Rongyu Zang

doi:10.7717/peerj.3946

Can 9q34.2 rs633862 polymorphism predict survival in epithelial ovarian cancer?

PeerJ. 2017 Nov 1:5:e3946. doi: 10.7717/peerj.3946. eCollection 2017.

Authors

Rong Jiang^#¹, Yuan Xu^#², Pan Wang^#¹, Xi Cheng³, Tingyan Shi^{1

2}, Rongyu Zang^{1

3}

Affiliations

¹ Ovarian Cancer Program, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Zhongshan Hospital, Fudan University, Shanghai, China.
² Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.
³ Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

^# Contributed equally.

Abstract

Objective: Our previous genome-wide association study (GWAS) identified that the ABO rs633862 variant in chromosome 9q34.2 was associated with the risk of epithelial ovarian cancer (EOC) in Chinese Han women. The aim of the present study was to evaluate its prognostic effect on EOC.

Methods: A total of 669 EOC patients were enrolled for the genotyping of rs633862 variant in 9q34.2. We used Kaplan-Meier survival curves, univariate and multivariate Cox proportional hazard models to evaluate the association of rs633862 with overall survival (OS) in EOC patients.

Results: We found that rs633862 variant AG/GG genotypes were significantly associated with a longer OS by using univariate Cox proportional hazards regression analysis, compared with the rs633862 AA genotype (HR = 0.69, 95% CI [0.49-0.98], p = 0.035), albeit with a boardline significance in the multivariate analysis. Similar findings were observed in the subgroup of high-grade serous ovarian carcinoma. Further expression quantitative trait loci (eQTL) analysis indicated that the rs633862 AA genotype was associated with an increased level of ABO mRNA expression (p = 1.8 × 10^-11).

Conclusions: Supplementary to the previous GWAS, our study provides additional evidence on the prognostic value of the 9q34.2 rs633862 variant in EOC patients, and this variant may function by regulating the ABO mRNA expression.

Keywords: ABO gene; Epithelial ovarian cancer; Single nucleotide polymorphism.

Grants and funding

This study was supported by the funds from the “Zhongshan Development Program” Recruitment (Grant No. 016), the National Science Fund for Young Scholars (Grant No. 81402142) and the Chinese Anti-Cancer Association (Grant No. HYXH1002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.