Novel O-linked carbohydrate chains in the cellulase complex (cellulosome) of Clostridium thermocellum. 3-O-Methyl-N-acetylglucosamine as a constituent of a glycoprotein

J Biol Chem. 1989 Jan 15;264(2):1027-35.

Abstract

Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols, namely D-Galp-beta(1----4)-D-GalOH and (formula; see text) The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / analogs & derivatives*
  • Acetylglucosamine / analysis
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cellulase*
  • Chromatography, High Pressure Liquid
  • Clostridium / enzymology*
  • Glucosamine / analogs & derivatives*
  • Glycoproteins*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Sequence Data
  • Multienzyme Complexes*
  • Oligosaccharides / isolation & purification*

Substances

  • Glycoproteins
  • Multienzyme Complexes
  • Oligosaccharides
  • methyl-2-acetamido-2-deoxy-D-glucopyranoside
  • Cellulase
  • Glucosamine
  • Acetylglucosamine