Solution scattering approaches to dynamical ordering in biomolecular systems

Biochim Biophys Acta Gen Subj. 2018 Feb;1862(2):253-274. doi: 10.1016/j.bbagen.2017.10.015. Epub 2017 Oct 26.

Abstract

Clarification of solution structure and its modulation in proteins and protein complexes is crucially important to understand dynamical ordering in macromolecular systems. Small-angle x-ray scattering (SAXS) and small-angle neutron scattering (SANS) are among the most powerful techniques to derive structural information. Recent progress in sample preparation, instruments and software analysis is opening up a new era for small-angle scattering. In this review, recent progress and trends of SAXS and SANS are introduced from the point of view of instrumentation and analysis, touching on general features and standard methods of small-angle scattering. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato.

Keywords: Ab-initio modeling; CV-SANS; Deuteration; Hybrid method; SEC-SANS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Computational Biology*
  • Equipment Design
  • Humans
  • Kinetics
  • Models, Biological*
  • Molecular Dynamics Simulation
  • Neutron Diffraction* / instrumentation
  • Protein Conformation
  • Proteins / chemistry
  • Proteins / metabolism*
  • Scattering, Small Angle*
  • Structure-Activity Relationship
  • X-Ray Diffraction* / instrumentation

Substances

  • Proteins