Effects of chronic alcohol consumption on DNA damage and immune regulation induced by the environmental pollutant dibenzo[a,l]pyrene in oral tissues of mice

J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2017 Oct 2;35(4):213-222. doi: 10.1080/10590501.2017.1391514. Epub 2017 Dec 4.

Abstract

Previously, we showed that oral application of the environmental pollutant dibenzo[a,l]pyrene (DB[a,l]P) induces oral tumors in mice. Thus, in the present investigation we examined the effect of alcohol on DB[a,l]P-induced DNA damage and immune regulation; we showed that alcohol (6.4% v/v in the diet, 35% of Calories) significantly enhanced the levels of (-)-anti-trans-DB[a,l]P-dA while decreased the levels of GSH in the mouse oral tissues. Analysis of RNA expression revealed that DB[a,l]P alone upregulates inflammatory genes while alcohol suppresses several markers of immune surveillance. Collectively, these results suggest that alcohol may enhance oral carcinogenesis induced by DB[a,l]P.

Keywords: Alcohol; DB[a,l]P; DNA adduct; immune regulation; oral cancer.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alcohol Drinking / adverse effects*
  • Alcohol Drinking / immunology
  • Alcoholism
  • Animals
  • Benzopyrenes / metabolism*
  • Carcinogenesis
  • DNA Damage*
  • Environmental Pollutants / metabolism*
  • Mice
  • Mouth / immunology
  • Mouth / metabolism*
  • Mouth Neoplasms

Substances

  • Benzopyrenes
  • Environmental Pollutants
  • dibenzo(a,l)pyrene