Beneficial Effect of Bis(Hinokitiolato)Zn Complex on High-fat Diet-induced Lipid Accumulation in Mouse Liver and Kidney

In Vivo. 2017 Nov-Dec;31(6):1145-1151. doi: 10.21873/invivo.11181.

Abstract

Background/aim: Metabolic syndrome-induced lifestyle-related diseases include diabetes mellitus (DM) and hypertension, and Zn-based compounds have effects on DM. We aimed to investigate the ameliorating effects of bis(hinokitiolato)Zn, [Zn(hkt)2] on lipid metabolism in the liver and kidney, histopathologically.

Materials and methods: We used a high-fat diet (HFD)-fed C57BL/6J mouse model and administered a diet containing 10-20 mg Zn/kg body weight (BW) or 20 mg pioglitazone/kg BW as the positive control. After the treatments, we collected blood, liver, and kidney samples and morphologically evaluated the mouse organs for fat accumulation.

Results: After a 4-month HFD administration, ectopic fat deposition was detected in the liver and kidney. Furthermore, Zn accumulation in the liver and kidney increased following [Zn(hkt)2] treatment, that reduced lipid accumulations and lipid toxicity in these tissues.

Conclusion: The results of this study suggest that [Zn(hkt)2] could be a novel anti-dyslipidaemia compound for treating diet-induced obesity.

Keywords: Zn complex; animal model; ectopic fat deposition; morphological analysis.

MeSH terms

  • Animals
  • Diet, High-Fat / adverse effects
  • Disease Models, Animal
  • Dyslipidemias / drug therapy*
  • Dyslipidemias / metabolism
  • Dyslipidemias / pathology
  • Humans
  • Kidney / drug effects
  • Kidney / metabolism*
  • Lipid Metabolism / drug effects
  • Liver / drug effects
  • Liver / metabolism*
  • Metabolic Syndrome / drug therapy
  • Metabolic Syndrome / metabolism
  • Metabolic Syndrome / pathology
  • Mice
  • Organometallic Compounds / administration & dosage*
  • Pioglitazone
  • Thiazolidinediones / administration & dosage
  • Zinc / administration & dosage

Substances

  • Organometallic Compounds
  • Thiazolidinediones
  • Zinc
  • Pioglitazone