Background/aim: To analyze the interaction between the human mesenchymal stem cells (hMSC) and the chick embryo chorioallantoic membrane (CAM), in order to assess the still obscure process of vasculogenesis.
Materials and methods: We implanted hMSC onto CAM and we analyzed the morphology and the immunohistochemical profile of CAM.
Results: hMSC adhered to CAM, few of them entered the chorionic epithelium and the mesoderm and developed a CD44-/Ki67- status. hMSC stimulated the CAM mesenchymal cells (cMSC) to acquire endothelial and pericyte-like features and to generate cord/capillary-like structures (CLS) in the chorionic epithelium and the mesoderm, but they also entered these structures (CD34+/SMA (smooth muscle actin)+ hMSC). Simultaneously, hMSC induced a process of sprouting angiogenesis in the mesoderm, CD105+ hMSC being identified in the proximity of the angiogenic areas.
Conclusion: hMSC and CAM establish a genuine hotspot of vasculogenesis, which may evolve to a valuable experimental model for this research field.
Keywords: Angiogenesis; chorioallantoic membrane; mesenchymal stem cells; pericyte-like; vasculogenesis.
Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.