In vitro amoebicidal and antioxidant activities of some Tunisian seaweeds

Olfa Chiboub; Leila Ktari; Ines Sifaoui; Atteneri López-Arencibia; Maria Reyes-Batlle; Mondher Mejri; Basilio Valladares; Manef Abderrabba; José E Piñero; Jacob Lorenzo-Morales

doi:10.1016/j.exppara.2017.10.012

In vitro amoebicidal and antioxidant activities of some Tunisian seaweeds

Exp Parasitol. 2017 Dec:183:76-80. doi: 10.1016/j.exppara.2017.10.012. Epub 2017 Nov 2.

Authors

Affiliations

¹ Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia; University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna Tenerife, Canary Islands, Spain; Laboratory of Blue Biotechnology and Aquatic Bioproducts, National Institute of Marine Sciences and Technologies (INSTM), 28, Rue du 2 mars 1934, 2035, Salammbô, Tunisia. Electronic address: olfachiboubt@gmail.com.
² Laboratory of Blue Biotechnology and Aquatic Bioproducts, National Institute of Marine Sciences and Technologies (INSTM), 28, Rue du 2 mars 1934, 2035, Salammbô, Tunisia.
³ Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia; University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna Tenerife, Canary Islands, Spain.
⁴ University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna Tenerife, Canary Islands, Spain.
⁵ Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia.

PMID: 29102681
DOI: 10.1016/j.exppara.2017.10.012

Abstract

Free-living amoebae of genus Acanthamoeba are opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans' infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitis and also disseminated infections. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the Tunisian coasts and belonging to the 3 phyla (brown, green and red algae). The aim was to quantify the Total Phenolic Compounds in different organic extract, to evaluate antioxidant capacity (DPPH and ABTS) and to study the antiprotozoal activity against A. castellanii Neff. The parasites have been inhibited by all extracts with an IC₅₀ ranged from 52,3±1.8 μg/mL for ethyl acetate extract, to 134,6±0.7 μg/mL for the hexanic one for the various species studied.

Keywords: Acanthamoeba; Antioxidant capacity; Antiparasitic activity; Biological activity; Tunisian seaweeds.

MeSH terms

Acanthamoeba castellanii / drug effects
Amebicides / isolation & purification
Amebicides / pharmacology*
Antioxidants / isolation & purification
Antioxidants / pharmacology*
Dose-Response Relationship, Drug
Free Radical Scavengers / isolation & purification
Free Radical Scavengers / pharmacology
Inhibitory Concentration 50
Mediterranean Sea
Phaeophyceae / chemistry
Phaeophyceae / physiology*
Phenols / isolation & purification
Phenols / pharmacology
Rhodophyta / chemistry
Rhodophyta / physiology*
Solvents
Tunisia
Ulva / chemistry
Ulva / physiology*

Substances

Amebicides
Antioxidants
Free Radical Scavengers
Phenols
Solvents