Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs

Biochem Biophys Res Commun. 2018 Jan 1;495(1):401-407. doi: 10.1016/j.bbrc.2017.10.173. Epub 2017 Nov 11.

Abstract

Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.

Keywords: Hypothalamus; LC-ESI-MS/MS; Mannosylation; Neocortex; Nucleotide sugar; UDP-mannose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain Chemistry*
  • Cell Line
  • Cells, Cultured
  • Humans
  • Male
  • Mannose / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Uridine Diphosphate Sugars / analysis*
  • Uridine Diphosphate Sugars / metabolism

Substances

  • Uridine Diphosphate Sugars
  • uridine diphosphate mannose
  • Mannose