Prevention of polydimethylsiloxane microsphere migration using a mussel-inspired polydopamine coating for potential application in injection therapy

PLoS One. 2017 Nov 2;12(11):e0186877. doi: 10.1371/journal.pone.0186877. eCollection 2017.

Abstract

The use of injectable bulking agents is a feasible alternative procedure for conventional surgical therapy. In this study, poly(dimethylsiloxane) (PDMS) microspheres coated with polydopamine (PDA) were developed as a potential injection agent to prevent migration in vocal fold. Uniform PDMS microspheres are fabricated using a simple fluidic device and then coated with PDA. Cell attachment test reveals that the PDA-coated PDMS (PDA-PDMS) substrate favors cell adhesion and attachment. The injected PDA-PDMS microspheres persist without migration on reconstructed axial CT images, whereas, pristine PDMS locally migrates over a period of 12 weeks. The gross appearance of the implants retrieved at 4, 8, 12 and 34 weeks indicates that the PDA-PDMS group maintained their original position without significant migration until 34 weeks after injection. By contrast, there is diffuse local migration of the pristine PDMS group from 4 weeks after injection. The PDA-coated PDMS microspheres can potentially be used as easily injectable, non-absorbable filler without migration.

MeSH terms

  • Animals
  • Bivalvia*
  • Coated Materials, Biocompatible*
  • Dimethylpolysiloxanes / chemistry*
  • Excipients
  • Indoles*
  • Injections, Subcutaneous
  • Male
  • Microscopy, Electron, Scanning
  • Microspheres*
  • Polymers*
  • Rabbits
  • X-Ray Microtomography

Substances

  • Coated Materials, Biocompatible
  • Dimethylpolysiloxanes
  • Excipients
  • Indoles
  • Polymers
  • polydopamine
  • baysilon

Grants and funding

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017M3A9B4032446), and a grant from the “GRRC” Project of the Gyeonggi Provincial Government, Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.