Fabrication of nitrogen- and phosphorous-doped carbon dots by the pyrolysis method for iodide and iron(III) sensing

Abstract

A facile and novel strategy to synthesize nitrogen- and phosphorous-doped carbon dots (NPCDs) by single step pyrolysis method is described here. Citric acid is used as carbon source and di-ammonium hydrogen phosphate is used as both nitrogen and phosphorous sources, respectively. Through the extensive study on optical properties, morphology and chemical structures of the synthesized NPCDs, it is found that as-synthesized NPCDs exhibited good excitation-dependent luminescence property, spherical morphology and high stability. The obtained NPCDs are stable in aqueous medium and possess a quantum yield of 10.58%. In this work, a new assay method is developed to detect iodide ions using the synthesized NPCDs. Here, the inner filter effect is applied to detect the iodide ion and exhibited a wide linear response concentration range (10-60 μM) with a limit of detection (LOD) of 0.32 μM. Furthermore, the synthesized NPCDs are used for the selective detection of iron(III) (Fe³⁺ ) ions and cell imaging. Fe³⁺ ions sensing assay shows a detection range from 0.2 to 30 μM with a LOD of 72 nM. As an efficient photoluminescence sensor, the developed NPCDs have an excellent biocompatibility and low cytotoxicity, allowing Fe³⁺ ion detection in HeLa cells.

Keywords: Fe3+ ions sensing; bioimaging; carbon dots; iodide sensing.