Abstract
We recently found that a unique subset of innate-like γδ T cells develops from the DN2a stage of the fetal thymus independently of the zinc-finger transcription factor B cell leukemia/lymphoma 11b (Bcl11b). Herein, we characterize these Bcl11b-independent γδ T cells in the periphery as CD5-NK1.1+ and Granzyme B+, and we show that they are capable of producing interferon (IFN)-γ upon T cell receptor stimulation without Ca2+ influx. In wild-type mice, these cells were sparse in lymphoid tissues but abundant in non-lymphoid tissues, such as the liver. Bcl11b-independent CD5-NK1.1+ γδ T cells appeared and contributed to early protection before Bcl11b-dependent CD5+NK1.1- γδ T cells following Listeria monocytogenes infection, resembling their sequential appearance during development in the thymus.
Keywords:
Bcl11b; DN2a; Granzyme; IFN-γ; IL-17A; Listeria monocytogenes; bacteria; host defense; innate immunity; γδ T cell.
Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
MeSH terms
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Animals
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Antigens, Ly / metabolism*
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CD5 Antigens / deficiency
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CD5 Antigens / genetics*
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Cells, Cultured
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Female
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Gene Expression
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Granzymes / metabolism
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Interferon-gamma / analysis
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Interferon-gamma / metabolism
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Interleukin-17 / analysis
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Interleukin-17 / metabolism
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Listeria monocytogenes / physiology
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Listeriosis / immunology
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Listeriosis / prevention & control
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Liver / immunology
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Liver / metabolism
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Liver / microbiology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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NK Cell Lectin-Like Receptor Subfamily B / metabolism*
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Phenotype
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Receptors, Antigen, T-Cell, gamma-delta / metabolism*
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Repressor Proteins / deficiency
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism*
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Thymus Gland / cytology
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Thymus Gland / immunology
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Thymus Gland / metabolism
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Tumor Suppressor Proteins / deficiency
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism*
Substances
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Antigens, Ly
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Bcl11b protein, mouse
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CD5 Antigens
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Interleukin-17
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Klrb1c protein, mouse
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NK Cell Lectin-Like Receptor Subfamily B
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Receptors, Antigen, T-Cell, gamma-delta
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Repressor Proteins
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Tumor Suppressor Proteins
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Interferon-gamma
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Granzymes