Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H10, Its carboxyl domain (H10-C) or globular domain (H10-G) to B-DNA

V R Machha; C G Mikek; S Wellman; E A Lewis

doi:10.1016/j.bbrep.2017.09.009

Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H1⁰, Its carboxyl domain (H1⁰-C) or globular domain (H1⁰-G) to B-DNA

Biochem Biophys Rep. 2017 Oct 13:12:158-165. doi: 10.1016/j.bbrep.2017.09.009. eCollection 2017 Dec.

Authors

V R Machha¹, C G Mikek², S Wellman³, E A Lewis²

Affiliations

¹ Division of Hematology, Departments of Internal Medicine and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
² Department of Chemistry, Mississippi State University, Mississippi, MS 39762, USA.
³ Department of Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS 39216, USA.

Abstract

Linker histones (H1) are the basic proteins in higher eukaryotes that are responsible for the final condensation of chromatin. In contrast to the nucleosome core histone proteins, the role of H1 in compacting DNA is not clearly understood. In this study ITC was used to measure the binding constant, enthalpy change, and binding site size for the interactions of H1⁰, or its C-terminal (H1⁰-C) and globular (H1⁰-G) domains to highly polymerized calf-thymus DNA at temperatures from 288 K to 308 K. Heat capacity changes, ΔC_p, for these same H1⁰ binding interactions were estimated from the temperature dependence of the enthalpy changes. The enthalpy changes for binding H1⁰, H1⁰-C, or H1⁰-G to CT-DNA are all endothermic at 298 K, becoming more exothermic as the temperature is increased. The ΔH for binding H1⁰-G to CT-DNA is exothermic at temperatures above approximately 300 K. Osmotic stress experiments indicate that the binding of H1⁰ is accompanied by the release of approximately 35 water molecules. We estimate from our naked DNA titration results that the binding of the H1⁰ to the nucleosome places the H1⁰ protein in close contact with approximately 41 DNA bp. The breakdown is that the H1⁰ carboxyl terminus interacts with 28 bp of linker DNA on one side of the nucleosome, the H1⁰ globular domain binds directly to 7 bp of core DNA, and shields another 6 linker DNA bases, 3 bp on either side of the nucleosome where the linker DNA exits the nucleosome core.

Keywords: CD; CD, Circular Dichroism; CT-DNA; CT-DNA, Calf Thymus DNA; Chromatin; H10; H10-C; H10-G; Histone; ITC; ITC, Isothermal Titration Calorimetry; Nucleosome.