Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria

Ann Magnuson; Tanai Cardona

doi:10.1007/978-1-4939-7411-5_9

Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria

Methods Mol Biol. 2018:1696:137-145. doi: 10.1007/978-1-4939-7411-5_9.

Authors

Ann Magnuson¹, Tanai Cardona²

Affiliations

¹ Department of Chemistry, Ångström Laboratory, Uppsala University, Uppsala, Sweden. ann.magnuson@kemi.uu.se.
² Department of Life Sciences, Faculty of Natural Sciences, Imperial College, London, UK.

PMID: 29086401
DOI: 10.1007/978-1-4939-7411-5_9

Abstract

The isolation of thylakoid membranes, including intact membrane protein complexes, from heterocysts of filamentous cyanobacteria such as Nostoc punctiforme, is described. Protocols for BN-PAGE/SDS-PAGE 2-D electrophoresis are not included. However, the chapter ends with advisory notes on sample preparation for blue-native PAGE of thylakoid membrane proteins, which can then be used together with any standard protocol.

Keywords: Cell disruption; Cyanobacteria; Heterocyst; Membrane proteins; Sonication.

MeSH terms

Bacterial Proteins / isolation & purification
Cell Fractionation / methods*
Cyanobacteria / cytology*
Cyanobacteria / metabolism
Membrane Proteins / isolation & purification
Nitrogen Fixation
Photosystem I Protein Complex / isolation & purification
Photosystem II Protein Complex / isolation & purification
Sonication
Thylakoids / metabolism*

Substances

Bacterial Proteins
Membrane Proteins
Photosystem I Protein Complex
Photosystem II Protein Complex