Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient

Kazusato Oikawa; Takuya Inomata; Yoshitoshi Hirao; Tadashi Yamamoto; Marouane Baslam; Kentaro Kaneko; Toshiaki Mitsui

doi:10.1007/978-1-4939-7411-5_6

Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient

Methods Mol Biol. 2018:1696:91-105. doi: 10.1007/978-1-4939-7411-5_6.

Authors

Kazusato Oikawa¹, Takuya Inomata², Yoshitoshi Hirao³, Tadashi Yamamoto³, Marouane Baslam^{1

2}, Kentaro Kaneko^{1

2}, Toshiaki Mitsui^{4

5}

Affiliations

¹ Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, 950-2181, Japan.
² Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan.
³ Biofluid Biomarker Center, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan.
⁴ Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, 950-2181, Japan. t.mitsui@agr.niigata-u.ac.jp.
⁵ Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan. t.mitsui@agr.niigata-u.ac.jp.

PMID: 29086398
DOI: 10.1007/978-1-4939-7411-5_6

Abstract

The Golgi apparatus is an endomembrane system organelle and has roles in glycosylation, sorting, and secretion of proteins in the secretory pathway. It has a central function in living organism and is also essential for plant growth. Proteomic approaches to identify the Golgi membrane proteins have been performed in cell suspension cultures and many Golgi membrane-associated proteins were found, whereas it has well established in rice seedling yet. In this chapter, our recent improving published methods for isolated rice Golgi membranes by floating through a discontinuous sucrose density gradient are provided in detail with proteomic analyses.

Keywords: Floating; Golgi membranes; Oryza sativa; Proteomic analysis; Sucrose density gradient.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Fractionation / methods*
Centrifugation, Density Gradient
Golgi Apparatus / metabolism*
Intracellular Membranes / metabolism*
Oryza / cytology*
Oryza / metabolism
Plant Proteins / analysis
Proteomics / methods

Substances

Plant Proteins