Illumina sequencing of the chloroplast genome of common ragweed (Ambrosia artemisiifolia L.)

Erzsébet Nagy; Géza Hegedűs; János Taller; Barbara Kutasy; Eszter Virág

doi:10.1016/j.dib.2017.10.009

Illumina sequencing of the chloroplast genome of common ragweed (Ambrosia artemisiifolia L.)

Data Brief. 2017 Oct 7:15:606-611. doi: 10.1016/j.dib.2017.10.009. eCollection 2017 Dec.

Authors

Erzsébet Nagy¹, Géza Hegedűs², János Taller¹, Barbara Kutasy¹, Eszter Virág¹

Affiliations

¹ University of Pannonia, Georgikon Faculty, Department of Plant Science and Biotechnology, Keszthely, Hungary.
² University of Pannonia, Georgikon Faculty, Department of Economic Methodology, Keszthely, Hungary.

Abstract

Common ragweed (Ambrosia artemisiifolia L.) is the most widespread weed and the most dangerous pollen allergenic plant in large areas of the temperate zone. Since herbicides like PSI and PSII inhibitors have their target genes in the chloroplast genome, understanding the chloroplast genome may indirectly support the exploration of herbicide resistance and development of novel control methods. The aim of the present study was to sequence and reconstruct for the chloroplast genome of A. artemisiifolia and establish a molecular dataset. We used an Illumina MiSeq protocol to sequence the chloroplast genome of isolated intact organelles of ragweed plants grown in our experimental garden. The assembled chloroplast genome was found to be 152,215 bp (GC: 37.6%) in a quadripartite structure, where 80 protein coding genes, 30 tRNA and 4 rRNA genes were annotated in total. We also report the complete sequence of 114 genes encoded in A. artemisiifolia chloroplast genome supported by both MIRA and Velvet de novo assemblers and ordered to Helianthus annuus L. using the Geneious software.

Keywords: Ambrosia artemisiifolia; Chloroplast genome; Common ragweed; Illumina sequencing; cpDNA.