Overview of Lasers for Flow Cytometry

Methods Mol Biol. 2018:1678:447-479. doi: 10.1007/978-1-4939-7346-0_19.

Abstract

Lasers are critical elements of all flow cytometers. Instrument capabilities are dictated by the wavelengths and characteristics of its laser sources. In this chapter, we review the lasers available for flow cytometry, and provide guidance in choosing laser wavelengths and characteristics to best match the analytical needs of biomedical research laboratories.

Keywords: Diode; Diode-pumped solid state; Flow cytometry; Laser.

Publication types

  • Review

MeSH terms

  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Humans
  • Lasers* / classification
  • Lasers* / standards