Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli

Darya O Ashcheulova; Lina V Efimova; Aliaksandr Ya Lushchyk; Aliaksei V Yantsevich; Alexander N Baikov; Alexandra G Pershina

doi:10.1016/j.pep.2017.10.011

Production of the recombinant antimicrobial peptide UBI_18-35 in Escherichia coli

Protein Expr Purif. 2018 Mar:143:38-44. doi: 10.1016/j.pep.2017.10.011. Epub 2017 Oct 21.

Authors

Darya O Ashcheulova¹, Lina V Efimova², Aliaksandr Ya Lushchyk³, Aliaksei V Yantsevich³, Alexander N Baikov², Alexandra G Pershina⁴

Affiliations

¹ Siberian State Medical University, 2, Moskovsky trakt, 634050 Tomsk, Russia; National Research Tomsk Polytechnic University, 30, Lenina Ave., Tomsk 634050, Russia.
² Siberian State Medical University, 2, Moskovsky trakt, 634050 Tomsk, Russia.
³ Institute of Bioorganic Chemistry, NAS of Belarus, 5/2, Kuprevich Str., 220141 Minsk, Belarus.
⁴ Siberian State Medical University, 2, Moskovsky trakt, 634050 Tomsk, Russia; National Research Tomsk Polytechnic University, 30, Lenina Ave., Tomsk 634050, Russia. Electronic address: allysyz@mail.ru.

PMID: 29066154
DOI: 10.1016/j.pep.2017.10.011

Abstract

Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI_18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI_18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI_18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.

Keywords: Antimicrobial peptides; Inclusion bodies; Ketosteroid isomerase; Size exclusion chromatography; Ubiquicidine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / metabolism*
Anti-Bacterial Agents / pharmacology
Antimicrobial Cationic Peptides / genetics
Antimicrobial Cationic Peptides / metabolism*
Chromatography, Gel
Cloning, Molecular
Escherichia coli / genetics*
Inclusion Bodies / chemistry
Inclusion Bodies / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism*
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Antimicrobial Cationic Peptides
Recombinant Fusion Proteins