Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae

Ying Zhao; Zhixiao Gao; Binnian Tian; Kai Bi; Tao Chen; Huiquan Liu; Jiatao Xie; Jiasen Cheng; Yanping Fu; Daohong Jiang

doi:10.1371/journal.pone.0185907

Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae

PLoS One. 2017 Oct 24;12(10):e0185907. doi: 10.1371/journal.pone.0185907. eCollection 2017.

Authors

Ying Zhao^{1

2}, Zhixiao Gao^{1

2}, Binnian Tian^{1

2}, Kai Bi^{1

2}, Tao Chen², Huiquan Liu³, Jiatao Xie^{1

2}, Jiasen Cheng^{1

2}, Yanping Fu², Daohong Jiang^{1

2}

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, P-R China.
² The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, P-R China.
³ State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Shanxi, P-R China.

Abstract

Clubroot caused by Plasmodiophora brassicae, is a severe disease of cruciferous crops that causes large hypertrophic galls in the roots. The plant microbiome is important for growth promotion and disease suppression. In this study, using 16S rRNA and internal transcribed spacer (ITS) sequencing techniques, we compared the endosphere microbiome of symptomatic and asymptomatic B. napus roots infected with P. brassicae collected from the same natural clubroot field. The results showed that the microbial population and its relative abundance in the asymptomatic roots was far higher than that in the symptomatic roots, and that many microorganisms in asymptomatic roots have biological control and plant growth promotion functions that may be related to clubroot symptoms. These results suggest the importance of the endosphere microbiome in clubroot disease and provide potential bio-control resources for its prevention.

Publication types

Comparative Study

MeSH terms

Brassica napus / parasitology*
Cercozoa / pathogenicity*
Cluster Analysis
Microbiota*
Plant Roots / parasitology*
Real-Time Polymerase Chain Reaction

Grants and funding

The research was financially supported by the earmarked fund for China Agriculture Research System (CARS-13) and the Programme of Introducing Talents of Discipline to Universities in China (the 111 project no. B14032). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.