A novel approach for monitoring a temperature distribution inside a tissue during thermoablation is presented in the paper. A thermal profile is measured using a set of serially connected thermistors each bypassed by a capacitor. This technique allows a two-wire and simultaneous multi-point measurements using a multi-frequency measurement of electrical impedance. It is shown that application signals of appropriately selected frequency allows simultaneous measurement of temperature at five distinct points. This technique can be utilized in the assisting of a thermoablation process, and in other applications based on resistive or capacitive sensors.