Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei

Wei Wang; Yumeng Chen; Dong-Zhi Wei

doi:10.1016/j.mimet.2017.10.006

Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei

J Microbiol Methods. 2017 Dec:143:63-65. doi: 10.1016/j.mimet.2017.10.006. Epub 2017 Oct 18.

Authors

Wei Wang¹, Yumeng Chen², Dong-Zhi Wei³

Affiliations

¹ State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.. Electronic address: wadexp@ecust.edu.cn.
² State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
³ State key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.. Electronic address: dzhwei@ecust.edu.cn.

PMID: 29055726
DOI: 10.1016/j.mimet.2017.10.006

Abstract

We developed a copper-mediated gene expression on-off control system and an unmarked genetic modification strategy based on the modified copper responsive promoter Ptcu1c from the filamentous fungus Trichoderma reesei. The molecular tool for the gene expression on-off control can be used to manipulate many biological processes in T. reesei.

Keywords: Copper responsive promoter; Cre/loxP; Filamentous fungus; Gene expression; Marker rescue; On-off control; Trichoderma reesei; Unmarked genetic modification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Copper / metabolism*
Fungi / drug effects*
Fungi / genetics
Gene Expression Regulation, Fungal / drug effects*
Genetics, Microbial / methods*
Molecular Biology / methods*
Trace Elements / metabolism*
Trichoderma / drug effects*
Trichoderma / genetics

Substances

Trace Elements
Copper