Lower mitochondrial dysfunction in survivor septic patients with mitochondrial DNA haplogroup JT
Enferm Infecc Microbiol Clin (Engl Ed). 2018 Nov;36(9):539-543.
doi: 10.1016/j.eimc.2017.08.011.
Epub 2017 Oct 18.
[Article in
English,
Spanish]
Affiliations
- 1 Intensive Care Unit, Hospital Universitario de Canarias, Santa Cruz de Tenerife, Spain. Electronic address: lorentemartin@msn.com.
- 2 Intensive Care Unit, Hospital Universitario Nuestra Señora Candelaria, Santa Cruz de Tenerife, Spain.
- 3 Departamento de Bioquímica y Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras (CIBERER) e Instituto de Investigación Sanitaria de Aragón, Universidad de Zaragoza, Zaragoza, Spain.
- 4 Intensive Care Unit, Hospital Clínico Universitario de Valencia, Valencia, Spain.
- 5 Intensive Care Unit, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain.
- 6 Intensive Care Unit, Hospital San Jorge de Huesca, Huesca, Spain.
- 7 Intensive Care Unit, Hospital Insular, Las Palmas de Gran Canaria, Spain.
- 8 Research Unit, Hospital Universitario de Canarias, Santa Cruz de Tenerife, Spain.
- 9 Departamento de Bioquímica y Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras (CIBERER), Instituto de Investitación Sanitaria de Aragón y Fundación ARAID, Universidad de Zaragoza, Zaragoza, Spain.
Abstract
Objective:
The comparison on mitochondrial function between severe septic patients and healthy control subjects according to mitochondrial deoxyribonucleic acid (mtDNA) haplogroup has not been previously reported; and this was the objective of the current study.
Methods:
Prospective, multicenter, observational study. We obtained blood samples from 198 severe septic patients at days 1, 4 and 8 of severe sepsis diagnosis and from 96 sex- and age-matched healthy controls to determine mtDNA haplogroup and platelet respiratory complex IV (CIV) specific activity. The endpoint of the study was 30-day mortality.
Results:
We included 198 severe septic patients (38 with mtDNA haplogroup JT and 160 with mtDNA haplogroup non-JT) and 96 healthy control subjects (16 with mtDNA haplogroup JT and 80 with mtDNA haplogroup non-JT). We have no found statistically significant differences in platelet CIV specific activity between healthy controls and survivor severe septic patients with mtDNA haplogroup JT at days 1, 4 and 8 of severe sepsis diagnosis; and the remaining severe septic patients showed lower platelet CIV specific activity than healthy controls with the same mtDNA haplogroup.
Conclusions:
The new finding of our study was that survivor severe septic patients and healthy controls with mtDNA haplogroup JT showed no different platelet Civ specific activity.
Keywords:
ADNmt; Citocromo c oxidasa; Cytochrome c oxidase; Mitochondria; Mitocondria; Mortalidad; Mortality; Sepsis; mtDNA.
Copyright © 2017 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.
Publication types
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Comparative Study
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Multicenter Study
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Observational Study
MeSH terms
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Adult
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Aged
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DNA, Mitochondrial / blood
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DNA, Mitochondrial / classification
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DNA, Mitochondrial / genetics*
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Electron Transport Complex IV / blood
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Electron Transport Complex IV / genetics
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Female
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Genetic Predisposition to Disease
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Haplotypes*
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Humans
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Male
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Middle Aged
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Mitochondria / physiology*
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Oxidative Phosphorylation
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Prognosis
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Prospective Studies
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Sepsis / blood
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Sepsis / genetics
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Sepsis / mortality
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Sepsis / physiopathology*
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Survivors
Substances
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DNA, Mitochondrial
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Electron Transport Complex IV