Structure and in vitro anticancer activity of sulfated O-polysaccharide from marine bacterium Poseidonocella pacifica KMM 9010T

Maxim S Kokoulin; Alexandra S Kuzmich; Anatoly I Kalinovsky; Eugene S Rubtsov; Lyudmila A Romanenko; Valery V Mikhailov; Nadezhda A Komandrova

doi:10.1016/j.carbpol.2017.09.052

Structure and in vitro anticancer activity of sulfated O-polysaccharide from marine bacterium Poseidonocella pacifica KMM 9010^T

Carbohydr Polym. 2017 Dec 15:178:406-411. doi: 10.1016/j.carbpol.2017.09.052. Epub 2017 Sep 18.

Authors

Maxim S Kokoulin¹, Alexandra S Kuzmich², Anatoly I Kalinovsky², Eugene S Rubtsov³, Lyudmila A Romanenko², Valery V Mikhailov⁴, Nadezhda A Komandrova²

Affiliations

¹ G.B Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation. Electronic address: maxchem@mail.ru.
² G.B Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.
³ Far Eastern Federal University, Suhanova St., 8, 690950, Vladivostok, Russian Federation.
⁴ G.B Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation; Far Eastern Federal University, Suhanova St., 8, 690950, Vladivostok, Russian Federation.

PMID: 29050611
DOI: 10.1016/j.carbpol.2017.09.052

Abstract

We presented the structure of the sulfated polysaccharide moiety and anticancer activity in vitro of the О-deacylated lipopolysaccharide (DPS) isolated from the marine bacterium Poseidonocella pacifica KMM 9010^T. The structure of O-polysaccharide was investigated by chemical methods along with ¹H and ¹³C NMR spectroscopy. The O-polysaccharide was built up of sulfated disaccharide repeating units consisted of d-rhamnose (d-Rhaр) and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdop): →7)-β-Kdoр4Ac5S-(2→3)-β-d-Rhaр2S-(1→. We demonstrated that the DPS from P. pacifica KMM 9010^T non-toxic for normal mouse epidermal cells (JB6 Cl41 cell line) and inhibited colony formation of human colorectal carcinoma HT-29, breast adenocarcinoma MCF-7 and melanoma SK-MEL-5 cells in a dose-dependent manner.

Keywords: Anticancer activity; Lipopolysaccharide; Marine bacterium; O-polysaccharide; Poseidonocella pacifica; Sulfated 3-deoxy-d-manno-oct-2-ulosonic acid.

MeSH terms

Animals
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Humans
Magnetic Resonance Spectroscopy
Mice
O Antigens / pharmacology*
Rhamnose
Rhodobacteraceae / chemistry*
Sulfates

Substances

Antineoplastic Agents
O Antigens
Sulfates
Rhamnose