Reconnectable fiberscopes for chronic in vivo deep-brain imaging

M S Pochechuev; I V Fedotov; O I Ivashkina; M A Roshchina; D V Meshchankin; D A Sidorov-Biryukov; A B Fedotov; K V Anokhin; A M Zheltikov

doi:10.1002/jbio.201700106

Reconnectable fiberscopes for chronic in vivo deep-brain imaging

J Biophotonics. 2018 Apr;11(4):e201700106. doi: 10.1002/jbio.201700106. Epub 2018 Feb 7.

Authors

M S Pochechuev^{1

2}, I V Fedotov^{1

3

4

5}, O I Ivashkina^{2

4}, M A Roshchina^{2

4}, D V Meshchankin¹, D A Sidorov-Biryukov^{1

2

4

5}, A B Fedotov^{1

2

4

5}, K V Anokhin^{2

4

6}, A M Zheltikov^{1

2

3

4

5}

Affiliations

¹ Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Moscow, Russia.
² Kurchatov Institute National Research Center, Moscow, Russia.
³ Department of Physics and Astronomy, Texas A&M University, College Station, Texas.
⁴ Russian Quantum Center, Skolkovo, Russia.
⁵ Kazan Quantum Center, A.N. Tupolev Kazan National Research Technical University, Kazan, Russia.
⁶ P.K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia.

PMID: 29045067
DOI: 10.1002/jbio.201700106

Abstract

Reconnectable bundles consisting of thousands of optical fibers are shown to enable high-quality image transmission, offering a platform for the creation of implantable fiberscopes for minimally invasive in vivo brain imaging. Experiments on various lines of transgenic mice verify the performance of this fiberscope as a powerful tool for chronic in vivo neuroimaging using genetically encoded calcium indicators, neuronal activity markers as well as axon growth regulators and brain-specific protein drivers in deep regions of live brain.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Axons / metabolism
Brain / cytology
Brain / diagnostic imaging*
Calcium / metabolism
Image Processing, Computer-Assisted
Male
Mice
Neurons / cytology
Optical Fibers*
Optical Imaging / instrumentation*
Organ Specificity
Time Factors

Substances

Calcium