ER Microsome Preparation in Arabidopsis thaliana

Verena Kriechbaumer

doi:10.1007/978-1-4939-7389-7_9

ER Microsome Preparation in Arabidopsis thaliana

Methods Mol Biol. 2018:1691:117-123. doi: 10.1007/978-1-4939-7389-7_9.

Author

Verena Kriechbaumer¹

Affiliation

¹ Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK. vkriechbaumer@brookes.ac.uk.

PMID: 29043673
DOI: 10.1007/978-1-4939-7389-7_9

Abstract

Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro.Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions.

Keywords: Arabidopsis; Endoplasmic reticulum; Enzyme assays; Microsomal fraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism
Cell Fractionation* / methods
Endoplasmic Reticulum / metabolism*
Microsomes / metabolism*

Substances

Arabidopsis Proteins

Grants and funding

BB/J004987/1/Biotechnology and Biological Sciences Research Council/United Kingdom