Aim: Glutaredoxin is a conserved oxidoreductase in eukaryotes and prokaryotes. This study aimed to determine the role of Grx3 in cell survival, iron homeostasis and the oxidative stress response in Candida albicans.
Materials & methods: A grx3Δ/Δ mutant was obtained using PCR-mediated homologs recombination. The function of Grx3 was investigated by a series of biochemical methods.
Results: Deletion of GRX3 impaired growth and cell cycle, disturbance of iron homeostasis and activated the oxidative stress response. Furthermore, disruption of GRX3 caused oxidative damage and growth defects of C. albicans.
Conclusion: Our findings provide new insights into the role of GRX3 in C. albicans.
Keywords: Candida albicans; Grx3; iron homeostasis; oxidative stress response.