An easy way to detect dengue virus using nanoparticle-antibody conjugates

Virology. 2018 Jan 1:513:85-90. doi: 10.1016/j.virol.2017.10.001. Epub 2017 Oct 14.

Abstract

The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.

Keywords: Biosensor; Fast detection; Infectious diagnostics.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Viral / metabolism*
  • Biosensing Techniques / methods*
  • Dengue / diagnosis*
  • Dengue Virus / isolation & purification*
  • Diagnostic Tests, Routine / methods*
  • Metal Nanoparticles*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Surface Plasmon Resonance

Substances

  • Antibodies, Viral