Abstract
The cysteine hydrolase, N-acylethanolamine acid amidase (NAAA) is a promising target for analgesic and anti-inflammatory drugs. Here, we describe the development of two unprecedented NAAA-reactive activity-based probes as research tools for application in the discovery of new inhibitors and for the in-depth characterization of NAAA in its cellular environment.
MeSH terms
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Amidohydrolases / antagonists & inhibitors
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Amidohydrolases / metabolism*
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Drug Evaluation, Preclinical
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Enzyme Inhibitors / pharmacology
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Humans
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Molecular Probes / chemical synthesis
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Molecular Probes / chemistry*
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Molecular Probes / metabolism*
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Molecular Structure
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Threonine / chemistry
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beta-Lactams / chemistry
Substances
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Enzyme Inhibitors
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Molecular Probes
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beta-Lactams
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Threonine
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Amidohydrolases
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NAAA protein, human